AdvanceBio N-Glycanase-plus (PNGase F), ≥10 U/mL. Enzyme (a recombinant form of PNGase F) releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Supplied as 5 vials of GKE-5010B (5 x 400 mU, ≥10 U/ml, 1mM EDTA). Includes 5x N-glycanase Reaction Buffer:100 mM sodium phosphate pH 7.5, 0.1% sodium azide; denaturation solution: 2% SDS, 1 M 2-mercaptoethanol; detergent solution: 15% detergent; 5x N-glycanase Tris reaction buffer: 50 mM Tris-HCl pH 8.0.
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